secondary antibody (Vector Laboratories)

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Vector Laboratories secondary antibody
Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 396 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+goat+anti+mouse+igm/Biotinylated+Goat+Anti-Mouse+IgM+Antibody%2C+mu+chain+specific/pm40473739-214-7-9
Average 93 stars, based on 396 article reviews

secondary antibody - by Bioz Stars, 2026-09

93/100 stars

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Blocking Assay:

Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies
Article Snippet: .. Biotinylated goat anti-mouse IgM, Mu chain specific secondary antibody (Vector Laboratories, Newark, CA, USA) diluted 1:500 in blocking solution (1F10), or biotinylated goat anti-mouse IgG diluted 1:1000 (AT100) or 1:3000 (7F2 and AT180) was added to the sections for 1 h at RT. .. ImmPRESS HRP horse anti-mouse IgG polymer (Vector Laboratories, Newark, CA, USA) diluted 1:10 was also added to the secondary antibody solution, except for mouse tissue sections immunostained with 7F2.

Article Title: A novel pThr217 tau monoclonal antibody reveals neuropathological heterogeneity in tauopathies.
Article Snippet: .. Biotinylated goat anti-mouse IgM, Mu chain specific secondary antibody (Vector Laboratories, Newark, CA, USA) diluted 1:500 in blocking solution (1F10), or biotinylated goat anti-mouse IgG diluted 1:1000 (AT100) or 1:3000 (7F2 and AT180) was added to the sections for 1 h at RT. .. ImmPRESS HRP horse anti-mouse IgG polymer (Vector Laboratories, Newark, CA, USA) diluted 1:10 was also added to the secondary antibody solution, except for mouse tissue sections immunostained with 7F2.

Immunoperoxidase Staining:

Article Title: Chronic Cyclodextrin Treatment of Murine Niemann-Pick C Disease Ameliorates Neuronal Cholesterol and Glycosphingolipid Storage and Disease Progression
Article Snippet: .. The following secondary antibodies and reagents were purchased for use in immunoperoxidase staining: biotinylated goat anti-mouse IgM (BA-2020), biotinylated goat anti-mouse IgG (BA-9200), biotinylated goat anti-rabbit IgG (BA-1000), Vectastain ABC kit (PK-4000), and DAB Peroxidase Substrate Kit (SK-4100) from Vector Laboratories (Burlingame, CA). .. The following secondary and tertiary antibodies were purchased for use in immunofluorescence: FITC-conjugated goat anti-mouse IgG (γ specific-Fc, 55517) from MP Biomedicals (Solon, OH); Alexa Fluor 633-conjugated goat anti-mouse IgM (μ chain, A21046), Alexa Fluor 546-conjugated streptavidin (S11225), and Alexa Fluor 488 Signal-Amplification Kit for FITC-conjugated probes (A11053) from Molecular Probes/Invitrogen (Carlsbad, CA).

Incubation:

Article Title: Intrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells
Article Snippet: .. After supernatants were removed, plates were incubated with biotinylated goat anti-mouse IgM, IgG3, IgG1, IgG2b, IgG2a, or IgA mAbs, as indicated, for 2 hours and, after washing, incubated with HRP-conjugated streptavidin followed by VECTASTAIN AEC (3-amino-9-ethylcarbazole) peroxidase substrate (SK-4200, Vector Laboratories). .. Individual ASC spots were enumerated using the CTL ImmunoSpot software (Cellular Technology).

Article Title: Immunomodulation Targeting Abnormal Protein Conformation Reduces Pathology in a Mouse Model of Alzheimer's Disease
Article Snippet: .. Bound antibody staining was performed with a secondary biotinylated goat anti-mouse IgM (mu chain specific, Vector Laboratories) incubated for 1 h at a 1∶500 dilution. .. First, primary antibody staining was revealed with 3,3′-diamonobenzidine (DAB Sigma-Aldrich) and nickel ammonium sulfate intensification.

Article Title: Intrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells.
Article Snippet: .. After supernatants were removed, plates were incubated with biotinylated goat anti-mouse IgM, IgG3, IgG1, IgG2b, IgG2a, or IgA mAbs, as indicated, for 2 hours and, after washing, incubated with HRP-conjugated streptavidin followed by VECTASTAIN AEC (3-amino-9-ethylcarbazole) peroxidase substrate (SK-4200, Vector Laboratories). .. Individual ASC spots were enumerated using the CTL ImmunoSpot software (Cellular Technology).

Clinical Proteomics:

Article Title: Pneumocystis murina lesions in lungs of experimentally infected Cd40l -/- mice.
Article Snippet: The Cd40l–/– mouse is a well-established model of X-linked hyper-immunoglobulin M (IgM) syndrome, an immunodeficiency disorder of human beings characterized by the lack of expression of the CD40 ligand (CD40L) on activated T-cells, predisposing to infections with opportunistic pathogens like Pneumocystis jirovecii.. The aim of our study was to describe the pulmonary lesions in Cd40l–/– mice experimentally infected with Pneumocystis murina, in comparison with naturally infected severe combined immunodeficient (SCID) mice.. Formalin-fixed paraffin-embedded lungs from 26 Cd40l–/–, 11 SCID, and 5 uninfected Cd40l–/– mice were examined by histology and immunohistochemistry for the presence of the pathogen and for leukocyte populations (CD3, CD4, CD45R/B220, CD8a, Iba-1, Ly-6G, CD206, MHC II, and NKp46/NCR1).

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secondary antibody (Vector Laboratories)

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